Not Simply Green: Nature-Based Solutions as a Concept and Practical Approach for Sustainability Studies and Planning Agendas in Cities

The concept of a nature-based solution (NBS) has been developed in order to operationalize an ecosystem services approach within spatial planning policies and practices, to fully integrate the ecological dimension, and, at the same time, to address current societal challenges in cities. It exceeds the bounds of traditional approaches that aim ‘to protect and preserve’ by considering enhancing, restoring, co-creating, and co-designing urban green networks with nature that are characterized by multifunctionality and connectivity. NBSs include the main ideas of green and blue infrastructure, ecosystem services, and biomimicry concepts, and they are considered to be urban design and planning tools for ecologically sensitive urban development. Nowadays, NBSs are on their way to the mainstream as part of both national and international policies. The successful implementation of NBSs in Europe and worldwide, which is becoming increasingly common, highlights the importance and relevance of NBS for sustainable and livable cities. This paper discusses the roles, development processes, and functions of NBSs in cities by taking Leipzig as a case study. Using data from interviews conducted from 2017 to 2019, we study the past and current challenges that the city faces, including the whole process of NBS implementation and successful realization. We discuss the main drivers, governance actors, and design options of NBSs. We highlight the ecosystem services provided by each NBS. We discuss these drivers and governance strategies by applying the framework for assessing the co-benefits of NBSs in urban areas in order to assess the opportunities and challenges that NBSs may have. This way, we are able to identify steps and procedures that help to increase the evidence base for the effectiveness of NBS by providing examples of best practice that demonstrate the multiple co-benefits provided by NBSs.Peer Reviewe

Lawns in Cities: From a Globalised Urban Green Space Phenomenon to Sustainable Nature-Based Solutions

This opinion paper discusses urban lawns, the most common part of open green spaces and urban green infrastructures. It highlights both the ecosystem services and also disservices provided by urban lawns based on the authors’ experience of working within interdisciplinary research projects on lawns in different cities of Europe (Germany, Sweden and Russia), New Zealand (Christchurch), USA (Syracuse, NY) and Australia (Perth). It complements this experience with a detailed literature review based on the most recent studies of different biophysical, social, planning and design aspects of lawns. We also used an international workshop as an important part of the research methodology. We argue that although lawns of Europe and the United States of America are now relatively well studied, other parts of the world still underestimate the importance of researching lawns as a complex ecological and social phenomenon. One of the core objectives of this paper is to share a paradigm of nature-based solutions in the context of lawns, which can be an important step towards finding resilient sustainable alternatives for urban green spaces in the time of growing urbanisation, increased urban land use competition, various user demands and related societal challenges of the urban environment. We hypothesise that these solutions may be found in urban ecosystems and various local native plant communities that are rich in species and able to withstand harsh conditions such as heavy trampling and droughts. To support the theoretical hypothesis of the relevance of nature-based solutions for lawns we also suggest and discuss the concept of two natures—different approaches to the vision of urban nature, including the understanding and appreciation of lawns. This will help to increase the awareness of existing local ecological approaches as well as an importance of introducing innovative landscape architecture practices. This article suggests that there is a potential for future transdisciplinary international research that might aid our understanding of lawns in different climatic and socio-cultural conditions as well as develop locally adapted (to environmental conditions, social needs and management policies) and accepted nature-based solutions.Peer Reviewe

Editorial for Special Issue “Nature-Based Solutions (NBS) in Cities and Their Interactions with Urban Land, Ecosystems, Built Environments and People: Debating Societal Implications”

Peer Reviewe

Urban Cemeteries—Places of Multiple Diversity and Challenges. A Case Study from Łódź (Poland) and Leipzig (Germany)

This article presents a pilot study investigating the multidimensional diversity of cemeteries as an important element of cultural heritage and green infrastructure within the urban landscape. We studied the state and diversity of nature, perceptions, and activities of visitors. As religion is an important aspect that differentiates cemeteries from each other, we studied a sample of four multi-confessional urban cemeteries in Łódź (Poland) and Leipzig (Germany) by using site observation and a questionnaire survey. We found that cemeteries are far undervalued as public green resources that can perform important functions in sociocultural life and the mental well-being of the general public, as the perceptions of silence- and contemplation-seeking visitors tell us. The perception of cemeteries depends on the level of secularization, varying from a sacrum sphere up to specific recreational and touristic opportunities; findings that should be considered by town planners when optimizing the cultural ecosystem services of green spaces.Peer Reviewe

Innovative e-Learning Training Modules to Improve Animal Welfare during Transport and Slaughter of Pigs: A Pretest–Posttest Study to Pre-Evaluate the General Didactical Concept

In addition to the information on the possession of a certificate of competence, there are no concrete obligations for repetitive training for personnel handling live animals at transport and slaughter. Deficiencies in the animal-welfare-friendly handling of pigs are known. The developed pilot modules “Handling of pigs” and “Electrical stunning” were tested in a pretest–posttest study in German and Romanian using questions of knowledge before and after the implementation of the modules. In this study, 45 and 46 datasets of participants could be analyzed. The mean percentages of correctly answered questions in the posttest increased by 5.6% in the module “Handling of pigs” and by 10.6% in the module “Electrical stunning”. A significant interaction was found for the language match and trend categories in the module “Handling of pigs”. No Romanian native speaker had a positive trend in this module. For both modules separately, participant education level significantly interacted with the language match and the presence or absence of a certificate of competence. Comparing the percentages of the correct given answers, significant interactions in the subgroups were more common in the module “Electrical stunning”. One question in “Electrical stunning” was correctly answered significantly more often in the posttest. Because of the positive mean trends of knowledge within this pre-evaluation, we assume the didactical concept was suitable for our target groups. Holders of a certificate of competence also gave more correct answers in the post-test. This underlines the importance of repetitive training. Differences in the trends of knowledge gain seem to be topic and experience related

Labeling of Anti-MUC-1 Binding Single Chain Fv Fragments to Surface Modified Upconversion Nanoparticles for an Initial in Vivo Molecular Imaging Proof of Principle Approach

In vivo optical Imaging is an inexpensive and highly sensitive modality to investigate and follow up diseases like breast cancer. However, fluorescence labels and specific tracers are still works in progress to bring this promising modality into the clinical day-to-day use. In this study an anti-MUC-1 binding single-chain antibody fragment was screened, produced and afterwards labeled with newly designed and surface modified NaYF4:Yb,Er upconversion nanoparticles as fluorescence reporter constructs. The MUC-1 binding of the conjugate was examined in vitro and in vivo using modified state-of-the-art small animal Imaging equipment. Binding of the newly generated upconversion nanoparticle based probe to MUC-1 positive cells was clearly shown via laser scanning microscopy and in an initial proof of principal small animal optical imaging approach

Bulk cell density and Wnt/TGFbeta signalling regulate mesendodermal patterning of human pluripotent stem cells

In vitro differentiation of human pluripotent stem cells (hPSCs) recapitulates early aspects of human embryogenesis, but the underlying processes are poorly understood and controlled. Here we show that modulating the bulk cell density (BCD: cell number per culture volume) deterministically alters anteroposterior patterning of primitive streak (PS)-like priming. The BCD in conjunction with the chemical WNT pathway activator CHIR99021 results in distinct paracrine microenvironments codifying hPSCs towards definitive endoderm, precardiac or presomitic mesoderm within the first 24 h of differentiation, respectively. Global gene expression and secretome analysis reveals that TGFß superfamily members, antagonist of Nodal signalling LEFTY1 and CER1, are paracrine determinants restricting PS progression. These data result in a tangible model disclosing how hPSC-released factors deflect CHIR99021-induced lineage commitment over time. By demonstrating a decisive, functional role of the BCD, we show its utility as a method to control lineage-specific differentiation. Furthermore, these findings have profound consequences for inter-experimental comparability, reproducibility, bioprocess optimization and scale-up.DFG/REBIRTHDFG/EXC62/1DFG/ZW 64/4-1DFG/MA 2331/16-1BMBF/13N12606BMBF/StemBANCCEU H2020/66872

Mutations in MITF and PAX3 Cause “Splashed White” and Other White Spotting Phenotypes in Horses

During fetal development neural-crest-derived melanoblasts migrate across the entire body surface and differentiate into melanocytes, the pigment-producing cells. Alterations in this precisely regulated process can lead to white spotting patterns. White spotting patterns in horses are a complex trait with a large phenotypic variance ranging from minimal white markings up to completely white horses. The “splashed white” pattern is primarily characterized by an extremely large blaze, often accompanied by extended white markings at the distal limbs and blue eyes. Some, but not all, splashed white horses are deaf. We analyzed a Quarter Horse family segregating for the splashed white coat color. Genome-wide linkage analysis in 31 horses gave a positive LOD score of 1.6 in a region on chromosome 6 containing the PAX3 gene. However, the linkage data were not in agreement with a monogenic inheritance of a single fully penetrant mutation. We sequenced the PAX3 gene and identified a missense mutation in some, but not all, splashed white Quarter Horses. Genome-wide association analysis indicated a potential second signal near MITF. We therefore sequenced the MITF gene and found a 10 bp insertion in the melanocyte-specific promoter. The MITF promoter variant was present in some splashed white Quarter Horses from the studied family, but also in splashed white horses from other horse breeds. Finally, we identified two additional non-synonymous mutations in the MITF gene in unrelated horses with white spotting phenotypes. Thus, several independent mutations in MITF and PAX3 together with known variants in the EDNRB and KIT genes explain a large proportion of horses with the more extreme white spotting phenotypes

Meeting Report: Validation of Toxicogenomics-Based Test Systems: ECVAM–ICCVAM/NICEATM Considerations for Regulatory Use

This is the report of the first workshop “Validation of Toxicogenomics-Based Test Systems” held 11–12 December 2003 in Ispra, Italy. The workshop was hosted by the European Centre for the Validation of Alternative Methods (ECVAM) and organized jointly by ECVAM, the U.S. Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM), and the National Toxicology Program (NTP) Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM). The primary aim of the workshop was for participants to discuss and define principles applicable to the validation of toxicogenomics platforms as well as validation of specific toxicologic test methods that incorporate toxicogenomics technologies. The workshop was viewed as an opportunity for initiating a dialogue between technologic experts, regulators, and the principal validation bodies and for identifying those factors to which the validation process would be applicable. It was felt that to do so now, as the technology is evolving and associated challenges are identified, would be a basis for the future validation of the technology when it reaches the appropriate stage. Because of the complexity of the issue, different aspects of the validation of toxicogenomics-based test methods were covered. The three focus areas include a) biologic validation of toxicogenomics-based test methods for regulatory decision making, b) technical and bioinformatics aspects related to validation, and c) validation issues as they relate to regulatory acceptance and use of toxicogenomics-based test methods. In this report we summarize the discussions and describe in detail the recommendations for future direction and priorities